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Image Search Results
Journal: Neuropsychiatric Disease and Treatment
Article Title: Shisandra Decoction Alleviates Parkinson’s Disease Symptoms in a Mouse Model Through PI3K/AKT/mTOR Signalling Pathway
doi: 10.2147/NDT.S476969
Figure Lengend Snippet: AKT and mTOR phosphorylation and relative abundance of p70S6K. ( A ) WB electrophoretogram. ( B ) and ( C ) Relative degree of AKT and mTOR phosphorylation. ( D ) Relative abundance of p70S6K. Use the Tukey HSD test statistical method. *P < 0.05, **P < 0.01 compared to control. # P < 0.05, ## P < 0.01 compared to model group. Δ P < 0.05, ΔΔ P < 0.01 compared to LY294002 group.
Article Snippet: MPTP (Shanghai Yuanye Biotechnology Co., Ltd., item number S31504-500mg); LY294002 (
Techniques: Phospho-proteomics, Control
Journal: Cell Death & Disease
Article Title: Microglia as modulators of exosomal alpha-synuclein transmission
doi: 10.1038/s41419-019-1404-9
Figure Lengend Snippet: Whole-cell lysates were analyzed by western blot. a Protein levels of LC3 ( n = 5, ** p = 0.0082), P62/SQSTM1 ( n = 5, **** p < 0.0001), Beclin ( n = 5, * p = 0.0101), phospho-AKT ( n = 5, *** p = 0.0004), total-AKT, phospho-mTOR ( n = 4, * p = 0.0183), and β-actin were shown in indicated cells. b Whole-cell lysates were immunoblotted against α-syn (oligomeric) ( n = 5, ** p = 0.0078); monomeric ( n = 4, * p = 0.0113) and β-actin for control. c The triple-stained image of P62/SQSTM1 (gray) with IBA1 (green) and DAPI (blue) in the SNpc
Article Snippet: The following primary antibodies were utilized: rabbit antibody to IBA1 (1:1000, 10904-1-AP, Proteintech), mouse antibody to tsg 101 (1:500, sc-7964, Santa cruz biotechnology), mouse antibody to CD63 (1:1000, ab59479, Abcam), rabbit antibody to Calnexin (1:1000, 10427-2-AP, Proteintech), rabbit antibody to α-syn (1:1000, ab138501, ab52168, Abcam), rabbit antibody to Oligomer (1:1000, AHB0052, ThermoFisher scientific), rabbit antibody to TH (1:1000, 25859-1-AP, Proteintech), rabbit antibody to LC3 (1:1000, 14600-1-AP, Proteintech), rabbit antibody to SQSTM1/P62 (1:1000, ab91526, Abcam), rabbit antibody to Beclin1 (1:1000, 11306-1-AP, Proteintech), rabbit antibody to phosphor-AKT (1:800, AP0655, Abclonal), rabbit antibody to AKT (1:800, A11030, Abclonal), rabbit antibody to
Techniques: Western Blot, Control, Staining
Journal: bioRxiv
Article Title: Argon inhibits autophagy and improves cerebral ischemia-reperfusion injury via PI3K/Akt/mTOR pathway
doi: 10.1101/2025.10.31.685964
Figure Lengend Snippet: A: western blot results showed that argon treatment significantly upregulated the levels of p-PI3K, p-Akt, and p-mTOR. B: Bar chart for A; C: western blot results of PI3K/Akt/mTOR pathway factors and autophagy factors in N 2, N 2 combined with SC79, and N 2 combined with MHY1485 group; D: Bar chart for C. E: western blot results of in argon, argon combined with MK2206 and argon combined with rapamycin groups. F: Bar chart for E. MHY: MHY1485; Rapa: rapamycin.
Article Snippet: The first antibodies included: P62 (Abcam, 1:1000, rabbit derived polyclonal antibody, ab91526), LC3 (Sigma Aldrich, 1:500, rabbit derived polyclonal antibody, ABC929), β-actin (MCE, 1:5000, mouse monoclonal antibody, HY-P80438), PI3K (Abcam, 1:1000, rabbit derived monoclonal antibody, ab302958), p-PI3K (Abcam, 1:1000, rabbit derived polyclonal antibody, ab138364), Akt (MCE, 1:2000, rabbit monoclonal antibody), p-Akt (MCE, 1:1000, rabbit monoclonal antibody, HY-P80276), mTOR (MCE, 1:1000, rabbit polyclonal antibody, HY-P80276),
Techniques: Western Blot